Hotspot

rpl22-rps19

LSC · 90764-90856 · 93 bp

Back to Aglaonema

Pi 0.0323
MarkerSeek score 71.00
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
93
variable_sites
3
pi
0.0323
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 GTACGCGGTTTACTCGAGTT GGAGAATTCGCACCTACTCG 50.0 55.0 58.0 58.2 200-203 bp 1.0 79.7
rpl22-rps19_p2 TTCTGTACGCGGTTTACTCG GGAGAATTCGCACCTACTCG 50.0 55.0 58.0 58.2 204-207 bp 1.0 79.6
rpl22-rps19_p3 GTACGCGGTTTACTCGAGTT GGGAGAATTCGCACCTACTC 50.0 55.0 58.0 57.8 201-204 bp 1.0 79.1
rpl22-rps19_p4 TTCTGTACGCGGTTTACTCG GGGAGAATTCGCACCTACTC 50.0 55.0 58.0 57.8 205-208 bp 1.0 79.1
rpl22-rps19_p5 GTACGCGGTTTACTCGAGTT TCGCACCTACTCGAACTTTC 50.0 50.0 58.0 57.7 193-196 bp 1.0 78.2