Hotspot

petA-psbJ

LSC · 69320-70434 · 1115 bp

Back to Aglaonema

Pi 0.0156

MarkerSeek score 69.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1115
variable_sites: 17
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGCGTTCAAGGTCTTTTGTT	TCCGGATTAGGCTCATCTCT	45.0	50.0	57.5	57.3	1229-1250 bp	1.0	73.4
petA-psbJ_p2	CGCGTTCAAGGTCTTTTGTT	CCGGATTAGGCTCATCTCTG	45.0	55.0	57.5	57.3	1228-1249 bp	1.0	72.9
petA-psbJ_p3	ACGCGTTCAAGGTCTTTTGT	TCCGGATTAGGCTCATCTCT	45.0	50.0	58.6	57.3	1230-1251 bp	1.0	72.1
petA-psbJ_p4	ACGCGTTCAAGGTCTTTTGT	CCGGATTAGGCTCATCTCTG	45.0	55.0	58.6	57.3	1229-1250 bp	1.0	71.7
petA-psbJ_p5	CGCGTTCAAGGTCTTTTGTT	TTCCGGATTAGGCTCATCTCT	45.0	47.6	57.5	58.0	1230-1251 bp	1.0	70.5