Hotspot

ycf4-cemA

LSC · 66438-67635 · 1198 bp

Back to Aglaonema

Pi 0.00858

MarkerSeek score 66.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 10
pi: 0.00858
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AAAAGCTGCGGAATTAGCCT	GATGTGTTTCGGGGTACTCC	45.0	55.0	58.2	58.0	1250-1278 bp	1.0	81.2
ycf4-cemA_p2	AAAAGCTGCGGAATTAGCCT	ATGTGTTTCGGGGTACTCCT	45.0	50.0	58.2	58.3	1249-1277 bp	1.0	78.6
ycf4-cemA_p3	AAAAAGCTGCGGAATTAGCC	GATGTGTTTCGGGGTACTCC	45.0	55.0	57.3	58.0	1251-1279 bp	1.0	77.3
ycf4-cemA_p4	AAAAGCTGCGGAATTAGCCT	GTGTTTCGGGGTACTCCTTT	45.0	50.0	58.2	57.4	1247-1275 bp	1.0	76.9
ycf4-cemA_p5	AAAGCTGCGGAATTAGCCTA	GATGTGTTTCGGGGTACTCC	45.0	55.0	57.3	58.0	1249-1277 bp	1.0	76.8