Hotspot

trnF-GAA-ndhJ

LSC · 52909-53937 · 1029 bp

Back to Aglaonema

Pi 0.0214

MarkerSeek score 75.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 21
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCTGAAAGTTGGATAGGCCG	50.0	55.0	58.1	58.3	1141-1153 bp	1.0	81.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CTGAAAGTTGGATAGGCCGG	50.0	55.0	58.1	58.3	1140-1152 bp	1.0	81.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTAAAACGT	50.0	45.0	58.1	57.6	1170-1182 bp	1.0	81.0
trnF-GAA-ndhJ_p4	CGATGCATGGAAAATGGTCG	CCTGAAAGTTGGATAGGCCG	50.0	55.0	57.9	58.3	1196-1208 bp	1.0	80.9
trnF-GAA-ndhJ_p5	CGATGCATGGAAAATGGTCG	CTGAAAGTTGGATAGGCCGG	50.0	55.0	57.9	58.3	1195-1207 bp	1.0	80.9