Hotspot

trnT-UGU-trnL-UAA

LSC · 50991-52023 · 1033 bp

Back to Aglaonema

Pi 0.0366
MarkerSeek score 81.90
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1033
variable_sites
37
pi
0.0366
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GGAAAAAGCCAGCTATCGGA ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1133-1177 bp 1.0 83.8
trnT-UGU-trnL-UAA_p2 AGGGAAAAAGCCAGCTATCG ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1135-1179 bp 1.0 83.8
trnT-UGU-trnL-UAA_p3 GGAAAAAGCCAGCTATCGGA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 1154-1198 bp 1.0 83.7
trnT-UGU-trnL-UAA_p4 AGGGAAAAAGCCAGCTATCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 1156-1200 bp 1.0 83.7
trnT-UGU-trnL-UAA_p5 GGAAAAAGCCAGCTATCGGA TAGCGTCTACCAATTTCGCC 50.0 50.0 58.0 58.1 1141-1185 bp 1.0 83.3