Hotspot

rps4-trnT-UGU

LSC · 50211-50915 · 705 bp

Back to Aglaonema

Pi 0.024

MarkerSeek score 71.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 14
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TACCAGAGCGCAATTGGTTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	58.1	57.9	780-887 bp	1.0	83.5
rps4-trnT-UGU_p2	TTACCAGAGCGCAATTGGTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	58.1	57.9	781-888 bp	1.0	83.5
rps4-trnT-UGU_p3	TTTACCAGAGCGCAATTGGT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	58.1	57.9	782-889 bp	1.0	83.5
rps4-trnT-UGU_p4	TACCAGAGCGCAATTGGTTT	GATGGTCATCGGTTCGACTC	45.0	55.0	58.1	58.2	749-856 bp	1.0	83.4
rps4-trnT-UGU_p5	TTACCAGAGCGCAATTGGTT	GATGGTCATCGGTTCGACTC	45.0	55.0	58.1	58.2	750-857 bp	1.0	83.4