Hotspot

trnE-UUC-trnT-GGU

LSC · 34619-35524 · 906 bp

Back to Aglaonema

Pi 0.0199

MarkerSeek score 74.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 17
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	978-993 bp	1.0	84.2
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	976-991 bp	1.0	84.2
trnE-UUC-trnT-GGU_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	957-972 bp	1.0	83.7
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	955-970 bp	1.0	83.7
trnE-UUC-trnT-GGU_p5	AGATGTCCTGAACCGCTAGA	AGTTTCGTGGAAAAAGCCCT	50.0	45.0	57.9	57.9	1007-1022 bp	1.0	83.6