Hotspot

trnY-GUA-trnE-UUC

LSC · 34380-34545 · 166 bp

Back to Aglaonema

Pi 0.0303

MarkerSeek score 69.60

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 4
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CGAATTTACAGTCCGTCCCC	TAGCGGTTCAGGACATCTCT	55.0	50.0	58.4	57.9	236-270 bp	1.0	81.3
trnY-GUA-trnE-UUC_p2	CGAATTTACAGTCCGTCCCC	TCTAGCGGTTCAGGACATCT	55.0	50.0	58.4	57.9	238-272 bp	1.0	81.3
trnY-GUA-trnE-UUC_p3	ACGAATTTACAGTCCGTCCC	TAGCGGTTCAGGACATCTCT	50.0	50.0	57.6	57.9	237-271 bp	1.0	81.1
trnY-GUA-trnE-UUC_p4	ACGAATTTACAGTCCGTCCC	TCTAGCGGTTCAGGACATCT	50.0	50.0	57.6	57.9	239-273 bp	1.0	81.1
trnY-GUA-trnE-UUC_p5	CGAATTTACAGTCCGTCCCC	GTCTAGCGGTTCAGGACATC	55.0	55.0	58.4	57.8	239-273 bp	1.0	80.7