Hotspot

rpoB-trnC-GCA

LSC · 29517-30981 · 1465 bp

Back to Aglaonema

Pi 0.0304
MarkerSeek score 72.10
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1465
variable_sites
43
pi
0.0304
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCCTCATTTTCATCCCGGAG GCGACACCCAGATTTGAACT 55.0 50.0 58.0 58.5 1523-1557 bp 1.0 80.9
rpoB-trnC-GCA_p2 CTCATTTTCATCCCGGAGCA GCGACACCCAGATTTGAACT 50.0 50.0 58.0 58.5 1521-1555 bp 1.0 80.8
rpoB-trnC-GCA_p3 CCCTCATTTTCATCCCGGAG TAAAGGATTTGCAGTCCCCC 55.0 50.0 58.0 57.5 1498-1532 bp 1.0 80.6
rpoB-trnC-GCA_p4 CTCATTTTCATCCCGGAGCA TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 57.5 1496-1530 bp 1.0 80.5
rpoB-trnC-GCA_p5 CCCTCATTTTCATCCCGGAG TTTGTTGATCAGGCGACACC 55.0 50.0 58.0 58.8 1535-1569 bp 1.0 78.6