Hotspot

rpoC1-rpoB

LSC · 26272-26297 · 26 bp

Back to Aglaonema

Pi 0.0385

MarkerSeek score 68.50

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TTACCTCTCCGACTACCTCC	ACGATCTTTGGCTCTGGAAC	55.0	50.0	57.3	57.9	214 bp	1.0	73.8
rpoC1-rpoB_p2	GACTACCTCCCCGTTAGGTA	ACGATCTTTGGCTCTGGAAC	55.0	50.0	57.3	57.9	204 bp	1.0	73.7
rpoC1-rpoB_p3	ACCTCCCCGTTAGGTAGAAT	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.1	57.9	200 bp	1.0	72.7
rpoC1-rpoB_p4	GTTGAGGAGAAACTGGTCCA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.1	57.9	157 bp	1.0	72.3
rpoC1-rpoB_p5	TGTTGAGGAGAAACTGGTCC	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.1	57.9	158 bp	1.0	72.3