Hotspot

trnQ-UUG-psbK

LSC · 8441-8823 · 383 bp

Back to Aglaonema

Pi 0.0317

MarkerSeek score 60.60

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 12
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	ATGGGTGCGGAAAGGAAAAT	AACTGCTTGAAGAAAGGGCA	45.0	45.0	58.1	57.9	1163-1176 bp	1.0	82.1
trnQ-UUG-psbK_p2	ATGGGTGCGGAAAGGAAAAT	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.1	57.9	1278-1291 bp	1.0	82.0
trnQ-UUG-psbK_p3	ATGGGTGCGGAAAGGAAAAT	GCGGACAAAAGGGAAAATGG	45.0	50.0	58.1	57.9	1468-1481 bp	1.0	81.5
trnQ-UUG-psbK_p4	ATGGGTGCGGAAAGGAAAAT	GGCGGACAAAAGGGAAAATG	45.0	50.0	58.1	57.9	1469-1482 bp	1.0	81.5
trnQ-UUG-psbK_p5	CTTAATGGGTGCGGAAAGGA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	57.9	57.9	1282-1295 bp	1.0	81.5