Hotspot

ycf15

LSC · 135748-135927 · 180 bp

Back to Ageratum

Pi 0.0222

MarkerSeek score 65.30

Primer available yes

Label ycf15

Feature Metadata

label_name: ycf15
feature_type: gene
region: LSC
length_bp: 180
variable_sites: 4
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15_p1	TGGCTCAGCGTGTTCTATTC	GTCCATTTTTCGGGGTCTCA	50.0	50.0	58.0	57.8	221-222 bp	1.0	79.8
ycf15_p2	TGGCTCAGCGTGTTCTATTC	TGTCCATTTTTCGGGGTCTC	50.0	50.0	58.0	57.8	222-223 bp	1.0	79.8
ycf15_p3	CTACGGATTCGAGCCATAGC	GTCCATTTTTCGGGGTCTCA	55.0	50.0	58.0	57.8	307-308 bp	1.0	79.4
ycf15_p4	CTACGGATTCGAGCCATAGC	TGTCCATTTTTCGGGGTCTC	55.0	50.0	58.0	57.8	308-309 bp	1.0	79.4
ycf15_p5	CCACACGATTTTCCCGATCT	GTCCATTTTTCGGGGTCTCA	50.0	50.0	58.0	57.8	271-272 bp	1.0	79.4