Hotspot

rps8

LSC · 79061-79468 · 408 bp

Back to Ageratum

Pi 0

MarkerSeek score 29.30

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 408
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	CAACCCGCCTCTTCTCTTTT	TGGAGGCACCAATCATTTTA	50.0	40.0	58.1	54.9	491 bp	1.0	54.3
rps8_p2	AACCCGCCTCTTCTCTTTTC	TGGAGGCACCAATCATTTTA	50.0	40.0	57.8	54.9	490 bp	1.0	53.7
rps8_p3	CAACCCGCCTCTTCTCTTTT	TGGAGGCACCAATCATTTTAA	50.0	38.1	58.1	55.7	491 bp	1.0	53.0
rps8_p4	AACCCGCCTCTTCTCTTTTC	TGGAGGCACCAATCATTTTAA	50.0	38.1	57.8	55.7	490 bp	1.0	52.4
rps8_p5	TTAGACAACCCGCCTCTTCT	TGGAGGCACCAATCATTTTA	50.0	40.0	58.4	54.9	496 bp	1.0	52.3