Hotspot

rps12

LSC · 96903-97180 · 278 bp

Back to Ageratum

Pi 0.0252

MarkerSeek score 67.00

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: LSC
length_bp: 278
variable_sites: 7
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	AGCGATTCACATGGCATCAT	GGAAGAAGGGAGGGGATTCT	45.0	55.0	58.0	58.1	429 bp	1.0	80.3
rps12_p2	TCCAGTCCCCTTACGAAACT	GGAAGAAGGGAGGGGATTCT	50.0	55.0	58.0	58.1	486 bp	1.0	80.2
rps12_p3	AGCGATTCACATGGCATCAT	AACGCGGAAAAGGATCCAAT	45.0	45.0	58.0	58.2	407 bp	1.0	79.9
rps12_p4	TCCAGTCCCCTTACGAAACT	AACGCGGAAAAGGATCCAAT	50.0	45.0	58.0	58.2	464 bp	1.0	79.7
rps12_p5	TCCAGTCCCCTTACGAAACT	ATTGAACGAGGAGCCGTATG	50.0	50.0	58.0	57.8	432 bp	1.0	79.3