Hotspot

atpF

LSC · 26390-27666 · 1277 bp

Back to Ageratum

Pi 0.00313

MarkerSeek score 60.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1277
variable_sites: 4
pi: 0.00313
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACAAAAAGAACTCTGTTCGA	ATTTCGTCGGCTTGAATGGT	35.0	45.0	53.0	58.2	1431-1434 bp	1.0	40.4
atpF_p2	ACAAAAAGAACTCTGTTCGA	GTCGGCTTGAATGGTTACCA	35.0	50.0	53.0	58.2	1426-1429 bp	1.0	40.4
atpF_p3	ACAAAAAGAACTCTGTTCGA	TCGTCGGCTTGAATGGTTAC	35.0	50.0	53.0	58.3	1428-1431 bp	1.0	40.4
atpF_p4	ACAAAAAGAACTCTGTTCGA	TTTCGTCGGCTTGAATGGTT	35.0	45.0	53.0	58.4	1430-1433 bp	1.0	40.4
atpF_p5	ACAAAAAGAACTCTGTTCGA	TTCGTCGGCTTGAATGGTTA	35.0	45.0	53.0	57.5	1429-1432 bp	1.0	40.4