Hotspot

accD

LSC · 56168-56689 · 522 bp

Back to Ageratum

Pi 0.0115

MarkerSeek score 63.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 522
variable_sites: 6
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTCATGCAAATATGGAGCAA	TGATCACTCGTTCTGGCATC	35.0	50.0	53.5	57.7	637 bp	1.0	42.4
accD_p2	TTTCATGCAAATATGGAGCA	TGATCACTCGTTCTGGCATC	35.0	50.0	53.5	57.7	638 bp	1.0	42.4
accD_p3	TTTCATGCAAATATGGAGCAA	TGATCACTCGTTCTGGCATC	33.3	50.0	54.4	57.7	638 bp	1.0	41.5
accD_p4	TTTTCATGCAAATATGGAGCA	TGATCACTCGTTCTGGCATC	33.3	50.0	54.4	57.7	639 bp	1.0	41.5
accD_p5	TTCATGCAAATATGGAGCAA	GATCACTCGTTCTGGCATCC	35.0	55.0	53.5	58.4	636 bp	1.0	41.4