Hotspot

ndhH-ndhA

SSC · 114548-116182 · 1635 bp

Back to Ageratum

Pi 0.00245

MarkerSeek score 44.90

Primer available yes

Label ndhH-ndhA

Feature Metadata

label_name: ndhH-ndhA
feature_type: igs
region: SSC
length_bp: 1635
variable_sites: 4
pi: 0.00245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-ndhA_p1	GGCCGCCGGGATTTATTAAT	CCTATAGGTTGACGCCACAA	50.0	50.0	58.5	57.3	1845-1846 bp	1.0	71.2
ndhH-ndhA_p2	GGCCGCCGGGATTTATTAAT	GTTGACGCCACAAATTCCAT	50.0	45.0	58.5	57.3	1838-1839 bp	1.0	70.9
ndhH-ndhA_p3	GGCCGCCGGGATTTATTAAT	GCCACAAATTCCATCCCCAA	50.0	50.0	58.5	58.7	1832-1833 bp	1.0	70.9
ndhH-ndhA_p4	GGCCGCCGGGATTTATTAAT	TGACGCCACAAATTCCATCC	50.0	50.0	58.5	58.8	1836-1837 bp	1.0	70.1
ndhH-ndhA_p5	GGCCGCCGGGATTTATTAAT	TTGACGCCACAAATTCCATC	50.0	45.0	58.5	57.0	1837-1838 bp	1.0	68.6