Hotspot

rps7-rps12

LSC · 96300-96902 · 603 bp

Back to Ageratum

Pi 0

MarkerSeek score 27.70

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: LSC
length_bp: 603
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TTCTTCTGCAGTACCTCGAC	TCAACAAGGGCGTTCTAGTG	50.0	50.0	57.0	57.8	658 bp	1.0	70.6
rps7-rps12_p2	TTCTTCTGCAGTACCTCGAC	GTCAACAAGGGCGTTCTAGT	50.0	50.0	57.0	57.8	659 bp	1.0	70.6
rps7-rps12_p3	TCTTCTGCAGTACCTCGACG	TCAACAAGGGCGTTCTAGTG	55.0	50.0	59.2	57.8	657 bp	1.0	69.1
rps7-rps12_p4	TCTTCTGCAGTACCTCGACG	GTCAACAAGGGCGTTCTAGT	55.0	50.0	59.2	57.8	658 bp	1.0	69.1
rps7-rps12_p5	TTTCTTCTGCAGTACCTCGAC	TCAACAAGGGCGTTCTAGTG	47.6	50.0	57.7	57.8	659 bp	1.0	68.3