Hotspot

petL-petG

LSC · 65054-65248 · 195 bp

Back to Ageratum

Pi 0

MarkerSeek score 18.80

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 195
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 18.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TCCAAGAATGCATTCGCTGA	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	57.9	58.0	812-813 bp	1.0	79.9
petL-petG_p2	TCCAAGAATGCATTCGCTGA	CAAAACCCGATGTCGTAGGT	45.0	50.0	57.9	57.9	776-777 bp	1.0	79.3
petL-petG_p3	TCCAAGAATGCATTCGCTGA	GGGTACAAAATGTCACGGGT	45.0	50.0	57.9	58.1	1012-1013 bp	1.0	79.0
petL-petG_p4	TCCAAGAATGCATTCGCTGA	TGGGTACAAAATGTCACGGG	45.0	50.0	57.9	58.1	1013-1014 bp	1.0	79.0
petL-petG_p5	TCCAAGAATGCATTCGCTGA	GTCACGGGTTCAAATCCTGT	45.0	50.0	57.9	58.1	1001-1002 bp	1.0	79.0