Hotspot

trnT-GGU-psbD

LSC · 30695-31935 · 1241 bp

Back to Ageratum

Pi 0
MarkerSeek score 63.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1241
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTAATCCCATGGTAACGCGT GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1319-1375 bp 1.0 82.8
trnT-GGU-psbD_p2 GTAATCCCATGGTAACGCGT GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1337-1393 bp 1.0 81.9
trnT-GGU-psbD_p3 AGTAATCCCATGGTAACGCG GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1320-1376 bp 1.0 81.4
trnT-GGU-psbD_p4 ATCCCATGGTAACGCGTAAG GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1316-1372 bp 1.0 81.4
trnT-GGU-psbD_p5 GTAATCCCATGGTAACGCGT ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1327-1383 bp 1.0 80.9