Hotspot

trnK-UUU-rps16

LSC · 4367-5127 · 761 bp

Back to Ageratum

Pi 0.00395
MarkerSeek score 61.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
761
variable_sites
3
pi
0.00395
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GGTATGGATGAATCCCTCGC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 954-955 bp 1.0 77.0
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 897-898 bp 1.0 76.5
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 896-897 bp 1.0 76.5
trnK-UUU-rps16_p4 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 983-984 bp 1.0 75.9
trnK-UUU-rps16_p5 CAGTCGTGGTCTTCCAAACT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 982-983 bp 1.0 75.9