Hotspot

ycf1

SSC · 108103-113154 · 5052 bp

Back to Ageratina

Pi 0.152

MarkerSeek score 77.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5052
variable_sites: 709
pi: 0.152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GATTTCCTTGAGACGGTCCC	AGTTGTAGAGCGATTCTGCG	55.0	50.0	58.0	58.1	578 bp	0.5	59.1
ycf1_p2	GGATTTCCTTGAGACGGTCC	AGTTGTAGAGCGATTCTGCG	55.0	50.0	58.0	58.1	579 bp	0.5	59.1
ycf1_p3	GGGATTTCCTTGAGACGGTC	AGTTGTAGAGCGATTCTGCG	55.0	50.0	58.0	58.1	580 bp	0.5	59.1
ycf1_p4	GAGACGGTCCCCAAATAACC	AGTTGTAGAGCGATTCTGCG	55.0	50.0	58.0	58.1	569 bp	0.5	59.0
ycf1_p5	CGCCTTTGAATACGGATGGA	AGTTGTAGAGCGATTCTGCG	50.0	50.0	58.1	58.1	543 bp	0.5	58.9