Hotspot

rpl2

LSC · 149250-150530 · 1281 bp

Back to Ageratina

Pi 0.104

MarkerSeek score 83.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1281
variable_sites: 113
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TGTAAAGACGAAGAAAGAAATTC	ACTTAATAGCATGGCGATACA	30.4	38.1	53.4	54.9	1339-1534 bp	1.0	50.3
rpl2_p2	TTTGTAAAGACGAAGAAAGAAA	ACTTAATAGCATGGCGATACA	27.3	38.1	52.2	54.9	1341-1536 bp	1.0	50.3
rpl2_p3	TTTTGTAAAGACGAAGAAAGAA	ACTTAATAGCATGGCGATACA	27.3	38.1	52.2	54.9	1342-1537 bp	1.0	50.3
rpl2_p4	TTTTTGTAAAGACGAAGAAAGA	ACTTAATAGCATGGCGATACA	27.3	38.1	52.2	54.9	1343-1538 bp	1.0	50.3
rpl2_p5	TTGTAAAGACGAAGAAAGAAATTC	ACTTAATAGCATGGCGATACA	29.2	38.1	54.1	54.9	1340-1535 bp	1.0	50.3
rpl2_p1	ACTTAATAGCATGGCGATACA	TGTAAAGACGAAGAAAGAAATTC	38.1	30.4	54.9	53.4	1339-1534 bp	1.0	50.3
rpl2_p2	ACTTAATAGCATGGCGATACA	TTTGTAAAGACGAAGAAAGAAA	38.1	27.3	54.9	52.2	1341-1536 bp	1.0	50.3
rpl2_p3	ACTTAATAGCATGGCGATACA	TTTTGTAAAGACGAAGAAAGAA	38.1	27.3	54.9	52.2	1342-1537 bp	1.0	50.3
rpl2_p4	ACTTAATAGCATGGCGATACA	TTTTTGTAAAGACGAAGAAAGA	38.1	27.3	54.9	52.2	1343-1538 bp	1.0	50.3
rpl2_p5	ACTTAATAGCATGGCGATACA	TTGTAAAGACGAAGAAAGAAATTC	38.1	29.2	54.9	54.1	1340-1535 bp	1.0	50.3