Hotspot

ycf15-rps12

IRa · 135709-136791 · 1083 bp

Back to Ageratina

Pi 0.296

MarkerSeek score 82.70

Primer available yes

Label ycf15-rps12

Feature Metadata

label_name: ycf15-rps12
feature_type: igs
region: IRa
length_bp: 1083
variable_sites: 294
pi: 0.296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-rps12_p1	GTACCGTGAGAGAAGCAAGG	TACTCCGACAGCATCTAGGG	55.0	55.0	58.0	58.0	1363 bp	0.5	59.8
ycf15-rps12_p2	GTACCGTGAGAGAAGCAAGG	AGCGATTCACATGGCATCAT	55.0	45.0	58.0	58.0	1445 bp	0.5	59.8
ycf15-rps12_p3	GAATAGAACACGCTGAGCCA	TACTCCGACAGCATCTAGGG	50.0	55.0	58.0	58.0	1754 bp	0.5	59.8
ycf15-rps12_p4	CCGGCTTAGTGAGTCCTTTC	TACTCCGACAGCATCTAGGG	55.0	55.0	58.0	58.0	1464 bp	0.5	59.8
ycf15-rps12_p5	GTACCGTGAGAGAAGCAAGG	TCTACAACGCACTAGAACGC	55.0	50.0	58.0	58.0	1400 bp	0.5	59.8