Hotspot

rps12-ycf15

IRb · 98709-99791 · 1083 bp

Back to Ageratina

Pi 0.296

MarkerSeek score 82.70

Primer available yes

Label rps12-ycf15

Feature Metadata

label_name: rps12-ycf15
feature_type: igs
region: IRb
length_bp: 1083
variable_sites: 294
pi: 0.296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ycf15_p1	TACTCCGACAGCATCTAGGG	GTACCGTGAGAGAAGCAAGG	55.0	55.0	58.0	58.0	1363 bp	0.5	59.8
rps12-ycf15_p2	AGCGATTCACATGGCATCAT	GTACCGTGAGAGAAGCAAGG	45.0	55.0	58.0	58.0	1445 bp	0.5	59.8
rps12-ycf15_p3	TACTCCGACAGCATCTAGGG	GAATAGAACACGCTGAGCCA	55.0	50.0	58.0	58.0	1754 bp	0.5	59.8
rps12-ycf15_p4	TACTCCGACAGCATCTAGGG	CCGGCTTAGTGAGTCCTTTC	55.0	55.0	58.0	58.0	1464 bp	0.5	59.8
rps12-ycf15_p5	TCTACAACGCACTAGAACGC	GTACCGTGAGAGAAGCAAGG	50.0	55.0	58.0	58.0	1400 bp	0.5	59.8