Hotspot

trnY-GUA-rpoB

LSC · 11768-12836 · 1069 bp

Back to Ageratina

Pi 0.147

MarkerSeek score 83.30

Primer available yes

Label trnY-GUA-rpoB

Feature Metadata

label_name: trnY-GUA-rpoB
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 146
pi: 0.147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-rpoB_p1	GGCGGGAGAAAAGATGGAAT	TCTGCCAAACCCTGATCAAC	50.0	50.0	57.9	58.1	1606-1701 bp	1.0	90.5
trnY-GUA-rpoB_p2	GGCGGGAGAAAAGATGGAAT	ACACGAACCCATATCCTTGC	50.0	50.0	57.9	58.0	2029-2124 bp	1.0	88.6
trnY-GUA-rpoB_p3	ACCCGTCCGAACTACAAAAC	ACACGAACCCATATCCTTGC	50.0	50.0	58.1	58.0	2152-2247 bp	1.0	87.8
trnY-GUA-rpoB_p4	CACCCGTCCGAACTACAAAA	ACACGAACCCATATCCTTGC	50.0	50.0	58.1	58.0	2153-2248 bp	1.0	87.8
trnY-GUA-rpoB_p5	GGATTCCCCGACCAATCAAA	ACACGAACCCATATCCTTGC	50.0	50.0	57.9	58.0	2271-2366 bp	1.0	87.4