Hotspot

trnS-GCU-trnC-GCA

LSC · 8675-9328 · 654 bp

Back to Ageratina

Pi 0.0891

MarkerSeek score 81.50

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 55
pi: 0.0891
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	TCAGAGATTCTCGCGAGTCT	50.0	50.0	58.2	58.0	1200-1253 bp	1.0	90.5
trnS-GCU-trnC-GCA_p2	AAATTAGGCTCGCCCCATTT	TCAGAGATTCTCGCGAGTCT	45.0	50.0	57.8	58.0	1755-1808 bp	1.0	89.1
trnS-GCU-trnC-GCA_p3	ACGGAAAGAGAGGGATTCGA	AGTGAGGGGAAGCTTTTTGG	50.0	50.0	58.2	58.0	988 bp	0.5	58.8
trnS-GCU-trnC-GCA_p4	AAATTAGGCTCGCCCCATTT	AGTGAGGGGAAGCTTTTTGG	45.0	50.0	57.8	58.0	1543 bp	0.5	58.7
trnS-GCU-trnC-GCA_p5	ACGGAAAGAGAGGGATTCGA	TAGTAATCCAAAGGCGGCAC	50.0	50.0	58.2	58.0	827 bp	0.5	58.5