Hotspot

rpoA

LSC · 73376-74395 · 1020 bp

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Pi 0.00098

MarkerSeek score 59.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 1
pi: 0.00098
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AGATTGTGCCAAAGATGCAA	ACACCTATGCCGCATAATGG	40.0	50.0	56.2	58.1	1223-1224 bp	1.0	64.9
rpoA_p2	AGATTGTGCCAAAGATGCAA	CGCATAATGGATGTCGACCT	40.0	50.0	56.2	57.8	1213-1214 bp	1.0	64.5
rpoA_p3	AGATTGTGCCAAAGATGCAA	CACCTATGCCGCATAATGGA	40.0	50.0	56.2	57.8	1222-1223 bp	1.0	64.3
rpoA_p4	AGATTGTGCCAAAGATGCAAA	ACACCTATGCCGCATAATGG	38.1	50.0	57.0	58.1	1223-1224 bp	1.0	63.0
rpoA_p5	AGATTGTGCCAAAGATGCAAA	CGCATAATGGATGTCGACCT	38.1	50.0	57.0	57.8	1213-1214 bp	1.0	62.6