Hotspot

rpl16

LSC · 76746-78249 · 1504 bp

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Pi 0.000665

MarkerSeek score 59.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1504
variable_sites: 1
pi: 0.000665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCTGGGG	AGACAAGAACCTTGTTTTTCCA	47.6	36.4	58.0	56.3	1659 bp	1.0	42.2
rpl16_p2	TCCAGAAAGAAAAACCTGGGG	ACCTTGTTTTTCCAAAAATCCCT	47.6	34.8	58.0	57.3	1651 bp	1.0	42.0
rpl16_p3	CCAGAAAGAAAAACCTGGGGT	AGACAAGAACCTTGTTTTTCCA	47.6	36.4	58.3	56.3	1658 bp	1.0	40.0
rpl16_p4	CCAGAAAGAAAAACCTGGGGT	ACCTTGTTTTTCCAAAAATCCCT	47.6	34.8	58.3	57.3	1650 bp	1.0	40.0
rpl16_p5	TCCAGAAAGAAAAACCTGGGG	AGACAAGAACCTTGTTTTTCCAA	47.6	34.8	58.0	57.0	1659 bp	1.0	40.0