Hotspot

rbcL-psaI

LSC · 55381-56306 · 926 bp

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Pi 0

MarkerSeek score 58.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.0	57.9	1083-1084 bp	1.0	79.3
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.2	57.9	1062-1063 bp	1.0	78.1
rbcL-psaI_p3	TGCAAATGGAGTGCTGAACT	GAAGTCATTGCAATTGCCGG	45.0	50.0	58.0	58.4	1086-1087 bp	1.0	77.0
rbcL-psaI_p4	TGCAAATGGAGTGCTGAACT	GGAAGTCATTGCAATTGCCG	45.0	50.0	58.0	58.4	1087-1088 bp	1.0	77.0
rbcL-psaI_p5	GCCGCAGCTTGTGAAATATG	GAAGTCATTGCAATTGCCGG	50.0	50.0	58.2	58.4	1065-1066 bp	1.0	75.8