Hotspot

trnC-GCA-rpoB

LSC · 17448-18586 · 1139 bp

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Pi 0.000878

MarkerSeek score 59.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 1
pi: 0.000878
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1293 bp	1.0	78.1
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1291 bp	1.0	78.1
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	1294 bp	1.0	78.1
trnC-GCA-rpoB_p4	GATTTTTGATCAGGCGGCAC	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1332 bp	1.0	78.0
trnC-GCA-rpoB_p5	GATTTTTGATCAGGCGGCAC	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1330 bp	1.0	78.0