Hotspot

trnD-GUC-psbM

LSC · 15062-16118 · 1057 bp

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Pi 0.000946
MarkerSeek score 59.30
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1057
variable_sites
1
pi
0.000946
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 AATAGGCATGCCATACACCC AGGCAGTAGGGACTAGAATGA 50.0 47.6 57.7 57.3 1216-1218 bp 1.0 64.2
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG AGGCAGTAGGGACTAGAATGA 50.0 47.6 58.5 57.3 1182-1184 bp 1.0 62.7
trnD-GUC-psbM_p3 AATAGGCATGCCATACACCC AAGGCAGTAGGGACTAGAATGA 50.0 45.5 57.7 58.0 1217-1219 bp 1.0 61.7
trnD-GUC-psbM_p4 AATAGGCATGCCATACACCC AGGCAGTAGGGACTAGAATGAA 50.0 45.5 57.7 58.0 1216-1218 bp 1.0 61.7
trnD-GUC-psbM_p5 AATAGGCATGCCATACACCC GGCAGTAGGGACTAGAATGAAC 50.0 50.0 57.7 57.9 1215-1217 bp 1.0 61.2