Hotspot

psbK-psbI

LSC · 6732-7013 · 282 bp

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Pi 0

MarkerSeek score 27.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 282
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	475 bp	1.0	78.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	426 bp	1.0	78.1
psbK-psbI_p3	ACCCGAAGCTTATGCCATTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	57.9	57.8	505 bp	1.0	77.4
psbK-psbI_p4	TTAGCGTTTGTTTGGCAAGC	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.2	57.8	435 bp	1.0	77.3
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	423 bp	1.0	76.1