Hotspot

ycf2

IRb · 88615-95481 · 6867 bp

Back to Agave

Pi 0.00025
MarkerSeek score 36.70
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
IRb
length_bp
6867
variable_sites
6
pi
0.00025
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 TGATTGAACAACCGGGATCA AGAAGGGGATAGGGCTAAGC 45.0 55.0 57.1 58.3 221-222 bp 1.0 73.1
ycf2_p2 TGATTGAACAACCGGGATCA AACAGGATCGTCCAGTTCCT 45.0 50.0 57.1 58.4 267-268 bp 1.0 72.0
ycf2_p3 TGATTGAACAACCGGGATCA AAACAGGATCGTCCAGTTCC 45.0 50.0 57.1 57.5 268-269 bp 1.0 71.0
ycf2_p4 TGATTGAACAACCGGGATCAA AGAAGGGGATAGGGCTAAGC 42.9 55.0 57.9 58.3 221-222 bp 1.0 70.9
ycf2_p5 TGATTGAACAACCGGGATCA ACAGGATCGTCCAGTTCCTA 45.0 50.0 57.1 57.5 266-267 bp 1.0 70.6