Hotspot

rpoC2

LSC · 16955-21109 · 4155 bp

Back to Agave

Pi 0.000436
MarkerSeek score 38.10
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4155
variable_sites
4
pi
0.000436
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 TCCGTTGGACATAGATCCCA ACCCGGATCTTCGTCCATAA 50.0 50.0 57.8 58.2 278 bp 1.0 77.5
rpoC2_p2 TCCGTTGGACATAGATCCCA TTACCCGGATCTTCGTCCAT 50.0 50.0 57.8 58.2 280 bp 1.0 77.5
rpoC2_p3 CGTTCCTGGCGGTATCAAAA ACCCGGATCTTCGTCCATAA 50.0 50.0 58.6 58.2 220 bp 1.0 74.6
rpoC2_p4 CGTTCCTGGCGGTATCAAAA TTACCCGGATCTTCGTCCAT 50.0 50.0 58.6 58.2 222 bp 1.0 74.6
rpoC2_p5 TCCGTTGGACATAGATCCCA TCTTACCCGGATCTTCGTCC 50.0 55.0 57.8 58.6 282 bp 1.0 74.4