Hotspot

rpl32-trnL-UAG

SSC · 115855-116490 · 636 bp

Back to Agave

Pi 0.0117
MarkerSeek score 50.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
636
variable_sites
13
pi
0.0117
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCAAAGTCAGTTTCTACCGG AGCGTGTCTACCGATTTCAC 47.6 50.0 58.2 58.0 740-743 bp 1.0 71.6
rpl32-trnL-UAG_p2 AGCAAAGTCAGTTTCTACCGG CAGCGTGTCTACCGATTTCA 47.6 50.0 58.2 58.0 741-744 bp 1.0 71.6
rpl32-trnL-UAG_p3 GCAAAGTCAGTTTCTACCGGA AGCGTGTCTACCGATTTCAC 47.6 50.0 58.2 58.0 739-742 bp 1.0 71.6
rpl32-trnL-UAG_p4 GCAAAGTCAGTTTCTACCGGA CAGCGTGTCTACCGATTTCA 47.6 50.0 58.2 58.0 740-743 bp 1.0 71.5
rpl32-trnL-UAG_p5 GCAAAGTCAGTTTCTACCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 56.7 58.0 739-742 bp 1.0 71.4