Hotspot

rps12-trnV-GAC

IRb · 101036-102660 · 1625 bp

Back to Agave

Pi 0.000176
MarkerSeek score 45.30
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1625
variable_sites
1
pi
0.000176
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA CGGGGGAGCAAGTCAAAATA 45.0 50.0 57.7 57.9 1781-1782 bp 1.0 77.0
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1720-1721 bp 1.0 76.7
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1724-1725 bp 1.0 76.7
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1719-1720 bp 1.0 76.7
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1722-1723 bp 1.0 75.7