Hotspot

ycf4-cemA

LSC · 62063-62916 · 854 bp

Back to Agave

Pi 0.001
MarkerSeek score 46.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
854
variable_sites
3
pi
0.001
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA ATGAGAGAGAGACCCACCAG 45.0 55.0 57.9 57.8 959 bp 1.0 78.3
ycf4-cemA_p2 CTTGCGCGTACCAATTGAAG ATGAGAGAGAGACCCACCAG 50.0 55.0 58.1 57.8 961 bp 1.0 78.0
ycf4-cemA_p3 TGCGCGTACCAATTGAAGTA GGGAGGGAAGTCAATGCTTT 45.0 50.0 57.9 57.8 912 bp 1.0 77.8
ycf4-cemA_p4 CTTGCGCGTACCAATTGAAG GGGAGGGAAGTCAATGCTTT 50.0 50.0 58.1 57.8 914 bp 1.0 77.5
ycf4-cemA_p5 GGAACCATTCCCTTGACTCG ATGAGAGAGAGACCCACCAG 55.0 55.0 58.3 57.8 1041 bp 1.0 76.9