Hotspot

trnD-GUC-trnT-GGU

LSC · 32399-32727 · 329 bp

Back to Agave

Pi 0.00839
MarkerSeek score 43.10
Primer available yes
Label trnD-GUC-trnT-GGU

Feature Metadata

label_name
trnD-GUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
329
variable_sites
5
pi
0.00839
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnT-GGU_p1 CCTCGCTGGGATTGTAGTTC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 464-465 bp 1.0 80.5
trnD-GUC-trnT-GGU_p2 CCTCGCTGGGATTGTAGTTC CCATGGCGTTACTCTACCAC 55.0 55.0 58.1 58.1 443-444 bp 1.0 80.1
trnD-GUC-trnT-GGU_p3 CCTCGCTGGGATTGTAGTTC TGGCGTTACTCTACCACTGA 55.0 50.0 58.1 58.1 440-441 bp 1.0 79.9
trnD-GUC-trnT-GGU_p4 TACACCTCGCTGGGATTGTA GAACCGATGACTTACGCCTT 50.0 50.0 58.1 58.0 468-469 bp 1.0 79.8
trnD-GUC-trnT-GGU_p5 TACACCTCGCTGGGATTGTA CCATGGCGTTACTCTACCAC 50.0 55.0 58.1 58.1 447-448 bp 1.0 79.4