Hotspot

psbM-trnE-UUC

LSC · 30570-31811 · 1242 bp

Back to Agave

Pi 0.00123
MarkerSeek score 49.10
Primer available yes
Label psbM-trnE-UUC

Feature Metadata

label_name
psbM-trnE-UUC
feature_type
igs
region
LSC
length_bp
1242
variable_sites
4
pi
0.00123
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnE-UUC_p1 AGAATGAACAGTGCAGTAGC CGAATCCCCGTTTTCTCCTT 45.0 50.0 55.8 57.9 1346-1347 bp 1.0 61.6
psbM-trnE-UUC_p2 AGAATGAACAGTGCAGTAGC TTTCGTAGTACCCTACCCCC 45.0 55.0 55.8 57.8 1375-1376 bp 1.0 61.2
psbM-trnE-UUC_p3 AGAATGAACAGTGCAGTAGC GGAAGTCGAATCCCCGTTTT 45.0 50.0 55.8 58.2 1352-1353 bp 1.0 61.0
psbM-trnE-UUC_p4 AGAATGAACAGTGCAGTAGC TGTCCTGAACCACTAGACGA 45.0 50.0 55.8 57.7 1316-1317 bp 1.0 60.2
psbM-trnE-UUC_p5 AGTGCGAGAATATTGACTTCCA CGAATCCCCGTTTTCTCCTT 40.9 50.0 57.5 57.9 1322-1323 bp 1.0 59.7