Hotspot

rrn16-rps16

Genome · 167008-172673 · 5666 bp

Back to Agapetes

Pi 0.0944

MarkerSeek score 63.80

Primer available yes

Label rrn16-rps16

Feature Metadata

label_name: rrn16-rps16
feature_type: igs
region: Genome
length_bp: 5666
variable_sites: 322
pi: 0.0944
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps16_p1	GGAATAACACCGTCCTCCAC	TCGTCCGCTTGACAATACTG	55.0	50.0	58.0	58.0	1898 bp	0.5	59.8
rrn16-rps16_p2	ACAAAGGAGAGCCTCTGTCT	TCGTCCGCTTGACAATACTG	50.0	50.0	58.0	58.0	1929 bp	0.5	59.7
rrn16-rps16_p3	GGAATAACACCGTCCTCCAC	TCCACCGAATTCAACGATCC	55.0	50.0	58.0	58.0	2431 bp	0.5	59.7
rrn16-rps16_p4	ACAAAGGAGAGCCTCTGTCT	TCCACCGAATTCAACGATCC	50.0	50.0	58.0	58.0	2462 bp	0.5	59.7
rrn16-rps16_p5	CGACTCGACAAGACAACGAT	TCGTCCGCTTGACAATACTG	50.0	50.0	58.0	58.0	2244 bp	0.5	59.6