Hotspot

rps15-trnN-GUU

Genome · 153875-158795 · 4921 bp

Back to Agapetes

Pi 0.0623

MarkerSeek score 79.50

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: Genome
length_bp: 4921
variable_sites: 300
pi: 0.0623
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	TTGCCTTACTCGCTACACAC	AAGACAACCAGAGACGGGTA	50.0	50.0	57.9	58.0	2463-2904 bp	1.0	89.1
rps15-trnN-GUU_p2	TTGCCTTACTCGCTACACAC	AATAAGCCGCTCCATGAAGG	50.0	50.0	57.9	58.0	2389-2830 bp	1.0	89.1
rps15-trnN-GUU_p3	TTGCCTTACTCGCTACACAC	GATTCTGACTCGATCGTCCG	50.0	55.0	57.9	58.0	1629 bp	0.5	59.2
rps15-trnN-GUU_p4	TTGCCTTACTCGCTACACAC	TCAGTGATTGGAGGTGGACT	50.0	50.0	57.9	58.0	2575 bp	0.5	59.2
rps15-trnN-GUU_p5	GGAATAACACCGTCCTCCAC	TAAACAAGTAGGAGTGGCGC	55.0	50.0	58.0	57.9	1816 bp	0.5	59.1