Hotspot

ndhG-ndhI

Genome · 148181-149501 · 1321 bp

Back to Agapetes

Pi 0.0177

MarkerSeek score 70.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: Genome
length_bp: 1321
variable_sites: 23
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCAATCCCAGGAAGA	ACCACTGTCAATAATTGGCGA	50.0	42.9	57.9	57.9	1462-1478 bp	1.0	73.4
ndhG-ndhI_p2	AAGACCCAATCCCAGGAAGA	ACCACTGTCAATAATTGGCG	50.0	45.0	57.9	56.4	1462-1478 bp	1.0	69.2
ndhG-ndhI_p3	ACCCAATCCCAGGAAGACTA	ACCACTGTCAATAATTGGCGA	50.0	42.9	57.3	57.9	1459-1475 bp	1.0	68.6
ndhG-ndhI_p4	AGACCCAATCCCAGGAAGAC	ACCACTGTCAATAATTGGCGA	55.0	42.9	58.7	57.9	1461-1477 bp	1.0	68.2
ndhG-ndhI_p5	GACCCAATCCCAGGAAGACT	ACCACTGTCAATAATTGGCGA	55.0	42.9	58.7	57.9	1460-1476 bp	1.0	68.2