Hotspot

ndhI-ndhG

Genome · 131978-133298 · 1321 bp

Back to Agapetes

Pi 0.0177

MarkerSeek score 70.40

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: Genome
length_bp: 1321
variable_sites: 23
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	ACCACTGTCAATAATTGGCGA	AAGACCCAATCCCAGGAAGA	42.9	50.0	57.9	57.9	1462-1478 bp	1.0	73.4
ndhI-ndhG_p2	ACCACTGTCAATAATTGGCG	AAGACCCAATCCCAGGAAGA	45.0	50.0	56.4	57.9	1462-1478 bp	1.0	69.2
ndhI-ndhG_p3	ACCACTGTCAATAATTGGCGA	ACCCAATCCCAGGAAGACTA	42.9	50.0	57.9	57.3	1459-1475 bp	1.0	68.6
ndhI-ndhG_p4	ACCACTGTCAATAATTGGCGA	AGACCCAATCCCAGGAAGAC	42.9	55.0	57.9	58.7	1461-1477 bp	1.0	68.2
ndhI-ndhG_p5	ACCACTGTCAATAATTGGCGA	GACCCAATCCCAGGAAGACT	42.9	55.0	57.9	58.7	1460-1476 bp	1.0	68.2