Hotspot

trnN-GUU-rps15

Genome · 122684-127604 · 4921 bp

Back to Agapetes

Pi 0.0634

MarkerSeek score 80.90

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: Genome
length_bp: 4921
variable_sites: 301
pi: 0.0634
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	AAGACAACCAGAGACGGGTA	TTGCCTTACTCGCTACACAC	50.0	50.0	58.0	57.9	2463-2904 bp	1.0	91.3
trnN-GUU-rps15_p2	AATAAGCCGCTCCATGAAGG	TTGCCTTACTCGCTACACAC	50.0	50.0	58.0	57.9	2389-2830 bp	1.0	91.2
trnN-GUU-rps15_p3	GATTCTGACTCGATCGTCCG	TTGCCTTACTCGCTACACAC	55.0	50.0	58.0	57.9	1629 bp	0.5	59.2
trnN-GUU-rps15_p4	TCAGTGATTGGAGGTGGACT	TTGCCTTACTCGCTACACAC	50.0	50.0	58.0	57.9	2575 bp	0.5	59.2
trnN-GUU-rps15_p5	TAAACAAGTAGGAGTGGCGC	GGAATAACACCGTCCTCCAC	50.0	55.0	57.9	58.0	1816 bp	0.5	59.1