Hotspot

rpoB-rpoA

Genome · 80182-84864 · 4683 bp

Back to Agapetes

Pi 0.102

MarkerSeek score 74.70

Primer available yes

Label rpoB-rpoA

Feature Metadata

label_name: rpoB-rpoA
feature_type: igs
region: Genome
length_bp: 4683
variable_sites: 417
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoA_p1	TTGCGGTCCTTCATAAGACG	GATTGCCTCCCAGAATCTCA	50.0	50.0	58.0	57.0	703 bp	0.5	51.8
rpoB-rpoA_p2	AGTGTCAGCTTATCCATGCG	GATTGCCTCCCAGAATCTCA	50.0	50.0	58.1	57.0	1024 bp	0.5	51.4
rpoB-rpoA_p3	AAGACGAATTTCCGCTCGAA	GATTGCCTCCCAGAATCTCA	45.0	50.0	57.9	57.0	689 bp	0.5	51.3
rpoB-rpoA_p4	ATTGACGGGTTAGTGTCAGC	GATTGCCTCCCAGAATCTCA	50.0	50.0	57.9	57.0	1035 bp	0.5	51.1
rpoB-rpoA_p5	TTCCTTAGACGACGATTGCG	GATTGCCTCCCAGAATCTCA	50.0	50.0	58.1	57.0	718 bp	0.5	51.1