Hotspot

trnfM-CAU-atpI

Genome · 66857-67255 · 399 bp

Back to Agapetes

Pi 0.0329
MarkerSeek score 68.10
Primer available yes
Label trnfM-CAU-atpI

Feature Metadata

label_name
trnfM-CAU-atpI
feature_type
igs
region
Genome
length_bp
399
variable_sites
13
pi
0.0329
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-atpI_p1 CCAGACTACTCTACTCCCCG TTTTGCAACTTTAGCTGCGG 60.0 45.0 58.0 57.9 474-480 bp 1.0 81.5
trnfM-CAU-atpI_p2 AAGGTTATGAGCCTTGCGAG TTTTGCAACTTTAGCTGCGG 50.0 45.0 58.0 57.9 497-503 bp 1.0 81.4
trnfM-CAU-atpI_p3 TTGCGAGCTACCAGACTACT TTTTGCAACTTTAGCTGCGG 50.0 45.0 58.2 57.9 484-490 bp 1.0 80.4
trnfM-CAU-atpI_p4 CTTGCGAGCTACCAGACTAC TTTTGCAACTTTAGCTGCGG 55.0 45.0 57.8 57.9 485-491 bp 1.0 80.2
trnfM-CAU-atpI_p5 TTATGAGCCTTGCGAGCTAC TTTTGCAACTTTAGCTGCGG 50.0 45.0 57.8 57.9 493-499 bp 1.0 79.9