Hotspot

trnP-UGG-trnW-CCA

Genome · 33511-33696 · 186 bp

Back to Agapetes

Pi 0.0301
MarkerSeek score 69.70
Primer available yes
Label trnP-UGG-trnW-CCA

Feature Metadata

label_name
trnP-UGG-trnW-CCA
feature_type
igs
region
Genome
length_bp
186
variable_sites
5
pi
0.0301
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-trnW-CCA_p1 TACAAAATGTCGCGGGTTCA GTTTTGGAGACCCACGTTCT 45.0 50.0 58.1 58.0 246-262 bp 1.0 84.3
trnP-UGG-trnW-CCA_p2 TACAAAATGTCGCGGGTTCA TTTTGGAGACCCACGTTCTG 45.0 50.0 58.1 58.0 245-261 bp 1.0 84.3
trnP-UGG-trnW-CCA_p3 CAAAATGTCGCGGGTTCAAA GTTTTGGAGACCCACGTTCT 45.0 50.0 57.9 58.0 244-260 bp 1.0 84.3
trnP-UGG-trnW-CCA_p4 TACAAAATGTCGCGGGTTCA AGGAAATGAAATCGCGCTCT 45.0 45.0 58.1 58.0 307 bp 0.5 58.8
trnP-UGG-trnW-CCA_p5 CAAAATGTCGCGGGTTCAAA AGGAAATGAAATCGCGCTCT 45.0 45.0 57.9 58.0 305 bp 0.5 58.7