Hotspot

ndhA

SSC · 121897-123972 · 2076 bp

Back to Agapanthus

Pi 0.00128
MarkerSeek score 58.40
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2076
variable_sites
4
pi
0.00128
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 ATGAACCCGGTCACCATAGG TGGGAGAAGTTGATCGTTGA 55.0 45.0 59.2 56.5 2210 bp 1.0 58.7
ndhA_p2 CCGGTCACCATAGGGAACAT TGGGAGAAGTTGATCGTTGA 55.0 45.0 59.2 56.5 2204 bp 1.0 58.7
ndhA_p3 ATTTATGAACCCGGTCACCA TGGGAGAAGTTGATCGTTGA 45.0 45.0 56.8 56.5 2214 bp 1.0 58.6
ndhA_p4 TTTATGAACCCGGTCACCAT TGGGAGAAGTTGATCGTTGA 45.0 45.0 56.8 56.5 2213 bp 1.0 58.6
ndhA_p5 CCGGTCACCATAGGGAACAT TGGGAGAAGTTGATCGTTGAA 55.0 42.9 59.2 57.2 2204 bp 1.0 56.7