Hotspot

rps15-ycf1

SSC · 125541-125933 · 393 bp

Back to Agapanthus

Pi 0.00515

MarkerSeek score 58.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 393
variable_sites: 3
pi: 0.00515
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CTTGTCGAATAGCCGAGATGA	ACGGCAGTCATTTCAGTATGT	47.6	42.9	57.6	57.7	484-489 bp	1.0	59.8
rps15-ycf1_p2	TCTTGTCGAATAGCCGAGATG	ACGGCAGTCATTTCAGTATGT	47.6	42.9	57.6	57.7	485-490 bp	1.0	59.8
rps15-ycf1_p3	GCCTCCTTATCTTGTTGGTGA	ACGGCAGTCATTTCAGTATGT	47.6	42.9	57.6	57.7	550-555 bp	1.0	59.7
rps15-ycf1_p4	AGCCTCCTTATCTTGTTGGTG	ACGGCAGTCATTTCAGTATGT	47.6	42.9	57.6	57.7	551-556 bp	1.0	59.6
rps15-ycf1_p5	TGTCGAATAGCCGAGATGAA	ACGGCAGTCATTTCAGTATGT	45.0	42.9	56.5	57.7	482-487 bp	1.0	58.4